A novel preparation method of smart responsive glass sphere based composite carriers was reported in this study. Firstly, random block copolymers were synthesized by free radical polymerization of N⁃isopropylacrylamide, hydroxypropyl methacrylate and 3⁃trimethoxysilypropyl methacrylate. Then, the copolymer solution was uniformly sprayed on the surfaces of glass spheres by means of a self⁃made bottom spray fluidized bed reactor, and the bonding between copolymers and glass spheres was fabricated by thermal annealing, so as to form the copolymer/glass sphere composite carriers. The coating effects of the smart copolymers on the surfaces of glass spheres were investigated, including characteristic functional groups, surface microstructure, biocompatibility and thermosensibility. The results show that the temperature⁃responsive copolymers can be linked to the surfaces of glass spheres by bottom⁃spray coating technology, and the copolymer layers with certain thickness can be formed on the sphere surfaces. The composite carriers have good biocompatibility, and can realize effective cell adhesion and spontaneous desorption by using its intelligent response characteristics.

The polyvinylidene fluoride (PVDF) as a matrix material was prepared for a bilirubin⁃adsorbed membrane. Firstly, PVDF microfiltration membrane was prepared by vapor⁃induced phase inversion method. The effects of polymer concentration, solvent and additive type on membrane properties and structure were investigated. It is found that PVDF microfiltration membrane has excellent performance and morphology with the PVDF concentration in the casting solution of 7%~8% in quality. When blend solvent of acetone and N, N⁃dimethylformamide and the additive of glycerol were selected, pure water flux is higher than others. Polyethyleneimine was further coated on the matrix to prepare a modified PVDF membrane. The results showed that the modified PVDF membrane had certain serum bilirubin removal ability.

The flavonoids from lactuca tatarica were extracted by solvent⁃extraction method. The optimum extraction condition was determined by single factor experiment combined with the RSM. The results showed that the optimum extraction conditions for Mengshan lettuce were ethanol volume fraction of 70%, liquid⁃solid ratio of 40 mL/g, water bath temperature of 74 ℃, water bath time of 3.2 h. Under this condition, the actual yield of flavonoids was 37.06 mg/g (dry weight), which was very close to the prediction value. The model can simulate and forecast the yield well. This experiment provided the theoretical basis for actual production operation.

The biosurfactant produced by Pseudomonas aeruginosa strain KO5-2-9 could reduce surface tension to 26.6 mN/m and emulsified diesel up to 72.6% with the critical micelle concentration of 85.82 mg/L. Fourier transform infrared spectrum of extracted biosurfactant indicated the key component was glycolipid. The surface tension (ST) and emulsification index (E₂₄) were taken as evaluation indexes to study on stability of surfactants. The results showed that compared with chemical surfactants, the biosurfactant had high surface activity and emulsifying ability in high temperature, high salinity and high alkalinity. The results of physical simulation experiment indicated that the biosurfactant could enhance oil recovery over 8% and would have a good prospect in microbial enhanced oil recovery.

Temperatureresponsive culture substrates containing poly(Nisopropylacrylamide) (PNIPAAm) have been developed as an effective substitute for enzymatic treatment to recover adherent cells, which can control cell attachment and detachment by changing temperature. However, not all PNIPAAmcontaining surfaces can be applicable to cell culture and recovery. That means adherent cells show accommodative selectivity for thermoresponsive substrates. In this study, different cells including HeLa cells, HEK293 cells, bone marrow mesenchymal stem cells (BMMSCs) and adiposederived stem cells (ADSCs) were cultured on the temperatureresponsive PNIPAAm copolymer films with different surface wettability, topography and film thickness. The experimental results demonstrate that various cells had their special thermoresponsive substrates, and the optimal copolymer films were selected for HeLa cells, HEK293 cells, BMMSCs and ADSCs respectively.